Electrochemical Studies of the Interaction of Clarithromycin with Bovine Serum Albumin
- Release time:2024-08-09
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Affiliation of Author(s):
化学与化工学院
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Journal:
Analytical Science
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Key Words:
中文关键字：克拉霉素；牛血清白蛋白；相互作用；电极反应，英文关键字：clarithromycin; bovine serum albumi; interaction;
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Abstract:
The interaction of clarithromycin (CAM) with bovine serum albumin (BSA) was investigated in pH 4.5 – 8.0 phosphate buffer solutions in which three irreversible reduction waves P1 、P2 and P3 of CAM appeared on linear–sweep voltammetry on a static dropping mercury working electrode. In the acidic media, with the addition of BSA into the CAM solution，a new electrochemically active complex was formed and there was interaction between the carbonyl group C=O in the C-9 position of CAM and BSA. It was found that electrostatic and hydrophobic forces played an important role in the binding reaction. However, new electrochemically non–active complexes were formed at physiological pH condition. The study showed that the formation constant and formation ratio of the interaction between CAM and BSA were 1.51 × 1012 and 3:1 for P2 wave, and 4.53 × 105 and 1:1 for P3 wave, respectively. The ion strength enhanced the hydrophobic interaction between CAM and BSA.
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Note:
董社英
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Co-author:
Chun-XiaXUE,Ting-LinHUANG
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First Author:
dongsheying
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Indexed by:
Journal paper
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Volume:
卷：24
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Issue:
期：08.9
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Page Number:
页：1087-1091
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Translation or Not:
no
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Date of Publication:
2008-09-01
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