liuyongjun
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- Professor
- Supervisor of Doctorate Candidates
- Supervisor of Master's Candidates
- Name (Pinyin):liuyongjun
- School/Department:环境与市政工程学院
- Education Level:Postgraduate (Postdoctoral)
- Contact Information:电话 : 029-82202520
- Degree:Doctoral degree
- Professional Title:Professor
- Status:Employed
- Academic Titles:中国沙产业协会专家组成员
- Other Post:陕西省九三学社“乡村振兴研究院”副院长
- Alma Mater:西北工业大学
- Discipline:Environmental Science
Municipal Engineering
Environmental Engineering
Other Contact Information
- Telephone：
- Email：
- Paper Publications
Cloning of Salt Stress Responsive cDNA From Wheat
Release time:2024-08-09 Hits:
- Affiliation of Author(s):环境与市政工程学院
- Journal:Acta Genetica Sinica
- Key Words:中文关键字：wheat; gene，英文关键字：
- Abstract:Abstract：Analysis of the gene expression differentiation in wheat (Triticum aestivum Baofeng 7228 ) under salt stress was actualized by DDRT-PCR and 27 differential cDNA fragments were obtained. The expression of SR07 fragment was induced obviously by salt and the nucleotide sequence homology of 87% between SR07 fragment and PIPs was observed. Further research showed that 561bp ORF present in SR07 fragment. Plant expression vector of pCAMBIA-SR07 was constructed and 3 transformants of tobacco (Nicotiana tobacum) mediated by Agrobacterium tunefaciens plasmid were obtained. The resistance of 3 transformants was further researched. The results showed that no significant difference（P>0.05）was observed between the control and the transformants in resistance of salt stress, but there was significant difference（P<0.05） between the control and the transformants in resistance of PEG and mannitol stress. Therefore, we speculate that the expression of SR07 fragment may play an important role in water regulation.
- Note:刘永军
- First Author:liuyongjun
- Indexed by:Journal paper
- Volume:卷：34
- Issue:期：9
- Page Number:页：842-850
- Translation or Not:no
- Date of Publication:2007-09-01