Simultaneous detection of enteric bacteria from surface waters by QPCR in comparison with conventional bacterial indicators

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• Affiliation of Author(s):环境与市政工程学院
• Journal:Environ Monit Assess
• Key Words:中文关键字：通用引物，病原细菌，QPCR，膜过滤，英文关键字：Universal primer，pathogenic bacterica, QPCR, Memer
• Abstract:A rapid quantitative polymerase chain reaction (QPCR) method was developed for simultaneous detection of enteric bacteria from surface waters by utilizing a pair of universal primers which targeted four bacteria strains, namely Shigella dysenteriae, Vibrio cholerae, Salmonella typhimurium,and Escherichia coli. It was estimated that the QPCR method had a 94% confidence, and a detection limit as 2.7 E. coli cells persample in undiluted DNA extracts. The QPCR method was applied for the bacteriological examination of several surface waters and comparison was made with the conventional bacteria indicators determined by conventional membrane filter (MF) method. As a result, the calibrator cell equivalents (CCE) determined by QPCR was 2.2 to five times of the total coliform CFU, and the characteristics of the bacterial quality of different waters could be well presented by the QPCR results with a higher sensitivity. The coefficient of variation (CV) of data obtained by QPCR was smaller than that by traditional MF method, indicating a more stable analysis result. The QPCR method could thus be used as a supplement of the conventional culture method formores ensitive detection of pathogenic enteric bacteria from water.
• Note:刘永军
• First Author:王晓昌,zhangchongmiao,liuyongjun
• Indexed by:Journal paper
• Volume:卷：158
• Issue:期：
• Page Number:页：535-544
• Translation or Not:no
• Date of Publication:2009-11-01