Cloning of Salt Stress Responsive cDNA From Wheat
发布时间：2024-08-09
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- 所属单位：
- 环境与市政工程学院
- 发表刊物：
- Acta Genetica Sinica
- 关键字：
- 中文关键字：wheat; gene，英文关键字：
- 摘要：
- Abstract：Analysis of the gene expression differentiation in wheat (Triticum aestivum Baofeng 7228 ) under salt stress was actualized by DDRT-PCR and 27 differential cDNA fragments were obtained. The expression of SR07 fragment was induced obviously by salt and the nucleotide sequence homology of 87% between SR07 fragment and PIPs was observed. Further research showed that 561bp ORF present in SR07 fragment. Plant expression vector of pCAMBIA-SR07 was constructed and 3 transformants of tobacco (Nicotiana tobacum) mediated by Agrobacterium tunefaciens plasmid were obtained. The resistance of 3 transformants was further researched. The results showed that no significant difference（P>0.05）was observed between the control and the transformants in resistance of salt stress, but there was significant difference（P<0.05） between the control and the transformants in resistance of PEG and mannitol stress. Therefore, we speculate that the expression of SR07 fragment may play an important role in water regulation.
- 备注：
- 刘永军
- 第一作者：
- 刘永军
- 论文类型：
- 期刊论文
- 卷号：
- 卷：34
- 期号：
- 期：9
- 页面范围：
- 页：842-850
- 是否译文：
- 否
- 发表时间：
- 2007-09-01