Simultaneous Detection of Enteric Bacteria by QPCR in Surface Waters Located in Urban Area
发布时间：2024-08-09
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- 所属单位：
- 环境与市政工程学院
- 发表刊物：
- Human and Ecological Risk Assessment: An International Journal
- 关键字：
- 中文关键字：检测；病原细菌；QPCR，英文关键字：Detection; pathogenic bacteria;QPCR
- 摘要：
- Universal primers that targeted four bacteria strains, namely Shigella dysenteriae, Vibrio cholerae, Salmonella typhimurium,and Escherichia coli. It was estimated that the QPCR method had a 94% confidence, and a detection limit as 2.7 Escherichia coli cells per sample in undiluted DNA extracts. The QPCR method was applied for the bacteriological examination of several surface waters in the urban area of Xi’an, China. As a result, the calibrator cell equivalents (CCE) determined by QPCR was 2.2 to 5 times of the total coliform CFU, and the characteristics of the bacterial quality of different waters could be well presented by the QPCR results with a higher sensitivity.
- 备注：
- 刘永军
- 第一作者：
- 刘永军
- 论文类型：
- 期刊论文
- 卷号：
- 卷：15
- 期号：
- 期：05
- 页面范围：
- 页：1033 - 1048
- 是否译文：
- 否
- 发表时间：
- 2009-05-01