Rapid detection of pathogenic bacteria in surface water by PCR with universal primer
发布时间：2024-08-09
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- 所属单位：
- 环境与市政工程学院
- 发表刊物：
- Int. J. Environment and Pollution
- 关键字：
- 中文关键字：通用引物；病原细菌；PCR，英文关键字：universal primer; pathogenic bacteria; polymerase
- 摘要：
- A rapid PCR method was developed by utilising a pair of universal primers which targeted four bacteria strains, namely Shigella dysenteriae, Vibrio cholerae, Salmonella typhimurium and Escherichia coli. The bacteria from several water bodies in the urban area of Xi’an City were detected by PCR, and the comparison was made with the conventional method. As a result, the detection limit of the DNA of bacteria was 250 ng/L and the sensibility of PCR detection was 27.5 cfu/100 mL. The PCR method established by this study provides a more reliable approach for the assessment of bacteriological risk of water environment.
- 备注：
- 刘永军
- 第一作者：
- 王晓昌,张崇淼,刘永军
- 论文类型：
- 期刊论文
- 卷号：
- 卷：38
- 期号：
- 期：1/2
- 页面范围：
- 页：166-179
- 是否译文：
- 否
- 发表时间：
- 2009-12-01