Zhang，Denitrification characteristics of a newly isolated indigenous aerobic denitrifying bacterium under oligotrophic conditions
发布时间：2024-08-09
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- 所属单位：
- 环境与市政工程学院
- 发表刊物：
- Water Science & Technology
- 关键字：
- 中文关键字：aerobic denitrification, Bacillus sp. JM10, nitrog，英文关键字：aerobic denitrification, Bacillus sp. JM10, nitrog
- 摘要：
- A novel indigenous bacterium, strain JM10, isolated from the oligotrophic Hei he reservoir was characterized and showed aerobic denitrification ability. JM10 was identified as Bacillus sp. by phylogenetic analysis of its 16S rRNA gene sequence. Strain JM10 displayed very high levels of activity in aerobic conditions, consuming over 94.3% NO3 -N (approximately 3.06 mg L1) with a maximum reduction rate 0.108 mg NO3 -N L1 hours1. Full-factorial Box–Behnken design and response surface methodology were employed to investigate the optimal nitrate degradation conditions. The optimum conditions for nitrate degradation, at a rate of 0.140 mg L1 hours1, were found to be an inoculum size of 16.3% v/v, initial pH of 7.6, C/N ratio of 7.4, and temperature of 27.4 WC, where the C/N ratio and temperature had the largest effect on the nitrate degradation rate. Strain JM10 was added into the water samples from Hei he reservoir and the total nitrogen and nitrate removal rates of the strain reached 66.5% and 100%, respectively. Therefore, our results demonstrate that the strain JM10 favored the bioremediation of oligotrophic reservoir.
- 备注：
- 苏俊峰
- 合写作者：
- 张凯,马放,郭琳,张丽娜
- 第一作者：
- 黄廷林,苏俊峰
- 论文类型：
- 期刊论文
- 卷号：
- 卷：72
- 期号：
- 期：7
- 页面范围：
- 页：1082-1088
- 是否译文：
- 否
- 发表时间：
- 2015-11-01