Microprofiles of activated sludge aggregates using microelectrodes in completely autotrophic nitrogen removal over nitrite (CANON) reactor

Release time:2024-08-09  Hits:

• Affiliation of Author(s):环境与市政工程学院
• Journal:Frontiers of Environmental Science & Engineering
• Key Words:中文关键字：好氧氨氧化菌；厌氧氨氧化菌；亚硝酸盐氧化菌；微电极；单级自养脱氮，英文关键字：aerobic ammonium-oxidizing bacteria, anaerobic amm
• Abstract:Microsensor measurements and fluorescence in situ hybridization (FISH) analysis were combined to investigate the microbial populations and activities in a lab-scale sequencing batch reactor (SBR) for completely autotrophic nitrogen removal over nitrite (CANON). Fed with synthetic wastewater rich in ammonia, the SBR removed 82.5 ± 5.4% of influent nitrogen and a maximum nitrogen-removal rate of 0.52 kg N m–3 d–1 was achieved. The FISH analysis revealed that aerobic ammonium-oxidizing bacteria (AerAOB) Nitrosomonas and anaerobic ammonium-oxidizing bacteria (AnAOB) dominated the community. To quantify the microbial activities inside the sludge aggregates, microprofiles were measured using pH, dissolved oxygen (DO), NH4+, NO2– and NO3– microelectrodes. In the outer layer of sludge aggregates (0–700 m), nitrite-oxidizing bacteria (NOB) showed high activity with 4.1 mol cm–3 h–1 of maximum production rate under the condition of DO concentration higher than 3.3 mg L–1. Maximum AerAOB activity was detected in the middle layer (depths around 1700 m) where DO concentration was 1.1 mg L–1. In the inner layer (2200–3500 m), where DO concentration was below 0.9 mg L–1, AnAOB activity was detected. We thus showed that information obtained from microscopic views can be helpful in optimizing the SBR performance.
• Note:吕永涛
• Co-author:陈宣,鞠恺,陈小强
• First Author:wangxudong,miaorui,wanglei,lvyongtao
• Indexed by:Journal paper
• Volume:卷：10
• Issue:期：2
• Page Number:页：390-398
• Translation or Not:no
• Date of Publication:2016-04-01