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Affiliation of Author(s):

环境与市政工程学院

Journal:

Desalination and Water Treatment

Key Words:

中文关键字：菌种分离；石油降解菌；生物修复石油污染土壤，英文关键字：Isolating; Hydrocarbon degraders;Biodegradation of

Abstract:

Inoculation of hydrocarbon-degraders will be a favorable feature for bioremediation of oil contaminated soil. In this work, enhanced biodegradation of crude oil was investigated using the process that inoculation of seven active hydrocarbon-degraders isolated from contaminated site in oil field. Preliminary results showed that isolated seven active bacterial strains were Acinetobacter SY21, Neisseria SY22, Plesiomonas SY23, Xanthomonas SY24, Azotobacter SY42, Flavobacterium SY43, and Pseudomonas SY44, respectively. Seven active degraders belong to gram-negative bacteria. They were capable of degrading crude oil and 6 other hydrocarbons (normally octane, paraffin wax, benzene, methylbenzene, phenol, and naphthalene) as carbon source and energy. Their biodegradation efficiencies of oil were higher than 80% after 8 days. The highest degradation was obtained for bacterial strain SY43 (Etwinia.sp), followed by a degradation rate for bacterial strain SY23 (Plesiomonas.sp). In addition, it was observed that a higher removal efficiency of TPH depend on large number of strains by more inoculums of isolates. Finally, it has been found that the adaptation of isolates was well because there was no decrease in the CFU even when concentration of crude oil was up to 34250 mg/kg. Moreover, the removal efficiency of TPH was up to 88.4% after active oil degrader was inocolumed while the corresponding removal efficiency of TPH was 1.4% without inoculums. These results indicated that both the SY43 and SY23 strains are easy to grow and establish a local ecology in the oil-contaminated soil. That is, biodegradation of crude oil in contaminated soil was enhanced by inoculating isolates.

Note:

徐金兰

Co-author:

邓海鑫,宋少华

First Author:

huangtinglin,xujinlan

Indexed by:

Journal paper

Volume:

卷：52

Issue:

期：

Page Number:

页：5126-5135

Translation or Not:

no

Date of Publication:

2014-07-01