liuyongjun
- Professor
- Supervisor of Doctorate Candidates
- Supervisor of Master's Candidates
- Name (Pinyin):liuyongjun
- School/Department:环境与市政工程学院
- Education Level:Postgraduate (Postdoctoral)
- Contact Information:电话 : 029-82202520
- Degree:Doctoral degree
- Professional Title:Professor
- Status:Employed
- Academic Titles:中国沙产业协会专家组成员
- Other Post:陕西省九三学社“乡村振兴研究院”副院长
- Alma Mater:西北工业大学
- Discipline:Environmental Science
Municipal Engineering
Environmental Engineering
Other Contact Information
- Telephone:
- Email:
- Paper Publications
环境水体中肠道病原细菌的定量PCR检测
Release time:2024-08-09 Hits:
- Affiliation of Author(s):环境与市政工程学院
- Journal:环境科学
- Key Words:中文关键字:地表水;通用引物;病原细菌;定量PCR;膜过滤,英文关键字:Surface water, universal primers, pathogenic bacte
- Abstract:采用通用引物,通过实时荧光定量PCR方法(QPCR),对西安市5处地表水体中肠道病原细菌的细胞密度进行4个月的连续检测,并将QPCR检测结果与滤膜法测得的大肠菌群CFU值进行比较分析。结果显示, QPCR法可信度为94%,最小检测值为每管未稀释DNA提取物含2.7个大肠杆菌细胞。5个水体(N=60)检测结果表明,QPCR检测结果是大肠菌群CFU的2.2~5倍。病原细菌的几何平均值范围,QPCR法在25~67000 CCE/100mL之间,MF法大肠菌群为3~45000 CFU/100mL之间。2种方法的离散和回归分析表明,QPCR检测结果与大肠菌群CFU显著正相关,秩相关系数为r=0.983。
- Note:刘永军
- First Author:王晓昌,zhangchongmiao,liuyongjun
- Indexed by:Journal paper
- Volume:卷:29
- Issue:期:(5)
- Page Number:页:23-28
- Translation or Not:no
- Date of Publication:2008-12-01