liuyongjun
|
- Professor
- Supervisor of Doctorate Candidates
- Supervisor of Master's Candidates
- Name (Pinyin):liuyongjun
- School/Department:环境与市政工程学院
- Education Level:Postgraduate (Postdoctoral)
- Contact Information:电话 : 029-82202520
- Degree:Doctoral degree
- Professional Title:Professor
- Status:Employed
- Academic Titles:中国沙产业协会专家组成员
- Other Post:陕西省九三学社“乡村振兴研究院”副院长
- Alma Mater:西北工业大学
- Discipline:Environmental Science
Municipal Engineering
Environmental Engineering
Other Contact Information
- Telephone：
- Email：
- Paper Publications
Rapid detection of pathogenic bacteria in surface water by PCR with universal primer
Release time:2024-08-09 Hits:
- Affiliation of Author(s):环境与市政工程学院
- Journal:Int. J. Environment and Pollution
- Key Words:中文关键字：通用引物；病原细菌；PCR，英文关键字：universal primer; pathogenic bacteria; polymerase
- Abstract:A rapid PCR method was developed by utilising a pair of universal primers which targeted four bacteria strains, namely Shigella dysenteriae, Vibrio cholerae, Salmonella typhimurium and Escherichia coli. The bacteria from several water bodies in the urban area of Xi’an City were detected by PCR, and the comparison was made with the conventional method. As a result, the detection limit of the DNA of bacteria was 250 ng/L and the sensibility of PCR detection was 27.5 cfu/100 mL. The PCR method established by this study provides a more reliable approach for the assessment of bacteriological risk of water environment.
- Note:刘永军
- First Author:王晓昌,zhangchongmiao,liuyongjun
- Indexed by:Journal paper
- Volume:卷：38
- Issue:期：1/2
- Page Number:页：166-179
- Translation or Not:no
- Date of Publication:2009-12-01