Biodegradation and detoxication of phenol by using free and immobilized cells

Release time:2024-08-09  Hits:

• Affiliation of Author(s):环境与市政工程学院
• Journal:Journal of Environmental Science and Health, Part A
• Key Words:中文关键字：苯酚降解；毒性试验；固定化细胞；自由细胞，英文关键字：Phenol biodegradation，detoxication，immobilizedcell
• Abstract:Strain XA05 and FG03 with high biodegradation activity of phenol were isolated from the activated sludge and phenol-contaminated soils in Northwest of China, respectively. DNA sequencing and homologous analysis of 16s rRNA gene identified that XA05 belonged to an Acinetobacter sp. and FG03 was closely related to the Sphingomonas sp. Cells of strain XA05 and FG03 were mixed at the ratio of 1:1, and polyvinyl alcohol (PVA) was used as a gel matrix to immobilize mixed cells by repeated freezing and thawing. Biodegradation was evaluated by determining phenol. Detoxication was evaluated by using Daphniamagna toxicity tests.Theremoval effciency of phenol and factors affecting phenol degradation werei nvestigated, the stability of the immobilized cells was alsoreported. Experiment alvalues indicated that both free cells and immobilized cells showed high phenol degradation effciencies, higher than 95% within 35 h with an initial concentration of 800 mg/L phenol, and the immobilized cells showed better performance than that of the suspended culture cells. These results indicate that immobilized Acinetobncter sp. XA05 and Sphingomonas sp.FG03 possesses a good application potential in the treatment of phenol-containing wastewater.
• Note:刘永军
• First Author:王晓昌,liuyongjun
• Indexed by:Journal paper
• Volume:卷：44
• Issue:期：(02)
• Page Number:页：130-136
• Translation or Not:no
• Date of Publication:2009-08-01