Study on the biotoxicity and mechanisms of typical pollutants in water by using the vicia faba bioassays

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• Affiliation of Author(s):环境与市政工程学院
• Journal:IWA - CoFXIAN2011 International Conference
• Key Words:中文关键字：Cadmium (Cd), phenol, biotoxicity, micronuclei, li，英文关键字：Cadmium (Cd), phenol, biotoxicity, micronuclei, li
• Abstract:Genotoxicity and cytotoxicity of Cadmium (Cd) and phenol in water were assessed by using the Vicia faba bioassays. The relationship between cell micronuclei and lipid peroxidation induced by Cd (1~100 mg/L) and phenol (0.5~50 mg/L) was also investigated. The result showed that Cd and phenol both induced micronuclei (MCN) and pycnosis formation in Vicia faba root tips. The MCN frequency and pycnosis increased significantly in the Vicia root cells under exposure to Cd and phenol in the concentration range of 5~100 mg/L and 2~50 mg/L, respectively. The mitoticindex (MI) decreased in a concentration-dependent manner and significant differences were found in the Vicia faba roots among the treatments and the control after exposure to 5~100 mg/L Cd and 2~50 mg/L phenol for 12h. Linear correlative relationships were identified between MCN frequency and lipid peroxidation in Vicia faba roots induced by Cd (R2 = 0.94) and phenol (R2 = 0.95), indicating that both Cd and phenol exposures could induce oxidative stress, cytotoxicity and genotoxicity in plant cells. The Vicia faba root MCN assays were thus proved to be simple, efficient and reproducible methods for the genotoxicity monitoring of water environment regarding Cd and phenol.
• Note:刘永军
• Co-author:闫志刚
• First Author:王晓昌,liuyongjun
• Indexed by:Journal paper
• Volume:卷：1
• Issue:期：1
• Page Number:页：324-330
• Translation or Not:no
• Date of Publication:2011-09-01